General Info

Welcome to the NSF/NIH-CCLI JMU Microarray Website!

Our primary activities are focused around hosting an annual “Microarray for Teaching” workshop here at James Madison University (Harrisonburg, VA).

Our second workshop was held from June 20-24, 2011.  To read more about this aspect of the JMU microarray project, follow the “Annual Workshop” tab at the top of this page. We are applying to NSF to continue this workshop and add a bacteriophage discovery and bioinformatics workshop at University of Mary Washington, starting in 2014.  Stay tuned!

2010 Workshop Participants and Leaders

We also serve as a GCAT array scanning facility.

To find out more about our scanning services, please contact us by posting a comment or sending an email (primary contact info is listed under the “Posts” tab.)

Microarray flow chart (cDNA-gDNA)

Experimental overview

Bordetella avium spotted PCR amplicon arrays In our lab, we use spotted 60-mer PCR amplicon arrays containing probes that represent approximately 2/3 of the ORFs in the B. avium genome.  There are two identical probes (spots) for each target ORF.  These spots are randomly placed throughout the array. Rather than mixing differentially labeled cDNA populations …

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phosphoramidite oligo synthesis chemistry

FAQs

Here is a list of frequently asked questions regarding microarray technology in general and the B. avium arrays specifically. As additional questions crop up, please submit them by posting comments at the bottom of this page.  I will answer them as they come! Thanks– Steph Stockwell Q:  Typically how many molecules of probe DNA are present in each …

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In situ oligonucleotide array fabrication by inkjet printing

A PoSAM array printer is being built by third and fourth year students in the JMU School of Engineering for our use.  It should be completed and functional within ~2 years.  This type of printer uses standard inkjet print heads to create oligonucleotide probes in situ on standard glass slides.  To learn more about this process, check out …

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science_daily

Interesting applications

Short and sweet: Summaries of interesting uses for microarrays Microarray technologies are often used to determine and assay biomarkers for cancer.  Here are some OTHER interesting ways in which microarray technology is impacting medicine. These links will take you to some short and sweet Science Daily articles.  These articles are written for a scientifically literate …

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